guanine nucleotide exchange activity of DH domain of human FGD2

 The DH domain has been deduced to be responsible for guanine nucleotide exchange in CDC42 to activate downstream factors. Our aim was to build a prokaryotic expression system for the DH domain and to examine its guanine nucleotide exchange activity toward CDC42 in vitro. A recombinant vector, which was successfully constructed based on pGEX-6P-1, was employed to express the DH …

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