Defucosylated Mouse-Dog Chimeric Anti-EGFR Antibody Exerts Antitumor Activities in Mouse Xenograft Models of Canine Tumors

The epidermal growth factor receptor (EGFR) contributes to tumor malignancy via gene amplification and protein overexpression. Previously, we developed an anti-human EGFR (hEGFR) monoclonal antibody, namely EMab-134, which detects hEGFR and dog EGFR (dEGFR) with high sensitivity and specificity. In this study, we produced a defucosylated mouse-dog chimeric anti-EGFR monoclonal antibody, namely E134Bf. In vitro analysis revealed that E134Bf highly exerted antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity against a canine osteosarcoma cell line (D-17) and a canine fibroblastic cell line (A-72), both of which express endogenous dEGFR.
Moreover, in vivo administration of E134Bf significantly suppressed the development of D-17 and A-72 compared with the control dog IgG in mouse xenografts. These results indicate that E134Bf exerts antitumor effects against dEGFR-expressing canine cancers and could be valuable as part of an antibody treatment regimen for dogs.

Detection of antibodies against three zoonotic Bartonella spp. and cross-reactivity among species and Coxiella burnetii in dogs and cats from Central Thailand

  • Dogs and cats are important reservoir hosts of bacterial zoonotic pathogens, especially the Proteobacteria, Bartonella spp., and Coxiella burnetii. Bartonella spp. and C. burnetii are Gram-negative intracellular bacteria causing cat-scratch disease and query fever, respectively. Despite these two pathogens being dangerous, studies of their seroprevalence and cross-reactivity are limited in Thailand.
  • The objectives of this study were to detect the seroprevalence of three zoonotic species of Bartonella and to evaluate cross-reactivity among Bartonella spp. and with C. burnetii. In total, 570 dog and cat serum samples were detected for antibodies against Bartonella spp. and C. burnetii using an indirect immunofluorescence antibody (IFA) test. At titer ≥ 1:64, tested serum that had a fluorescent intensity score ≥ 2 was interpreted as positive. Additionally, possible factors related to the seroprevalence were analyzed consisting of sex, breed, age, residing area, and ectoparasite control.
  • Overall, the seroprevalence levels of Bartonella spp. and C. burnetii were 13.16% and 1.23%, respectively. All antigens of Bartonella were reacted to sera (1.23-7.72%), furthermore, both phases of C. burnetii were revealed in sera (0.35-1.05%). Interestingly, there was a poor agreement of cross-reactivity among Bartonella spp. at 5.56-8.70%, while cross-reactivity between Bartonella spp. and C. burnetii also showed poor agreement (2.80%). It is suggested that dogs and cats are important reservoirs of Bartonella spp., even in animals with ectoparasite control.
  • The Bartonella seroprevalence was high in pure-breed animals with ectoparasite control, reflecting that Bartonella spp. infections can occur in owned, well-cared-for, and asymptomatic dogs and cats.

Serosurvey of anti- Leishmania ( Leishmaniainfantum antibodies in hunting dogs and hunters in Brazil

Background and aim: Although wild boar hunting activities and the hunting dog trade in the Atlantic Forest and Cerrado biomes of Brazil overlap both with endemic and with non-endemic areas for visceral leishmaniasis, no study to date has focused on Leishmania spp. exposure among hunting dogs and hunters. The aim of the present study was to assess the presence of Leishmania spp. antibodies in hunting dogs and hunters in different anthropized areas of two Brazilian biomes.
Materials and methods: Blood samples were collected from 170 hunting dogs and 46 hunters between October 2016 and May 2018. The presence of antibodies against Leishmania spp. in hunting dogs was screened through a dual-path platform immunochromatographic test (DPP rapid test; Bio-Manguinhos/Fundação Oswaldo Cruz, Rio de Janeiro, Brazil) and in hunters through an rK39-based rapid immunochromatographic test. Both tests were used in accordance with Brazilian Ministry of Health recommendations.
Results: Overall, although antibodies were detected through the immunochromatographic test in 3/170 (0.02%) of these female asymptomatic hunting dogs, all living in anthropized areas of the Atlantic Forest biome in South Brazil, no sample was confirmed through the enzyme-linked immunosorbent assay. All the hunters were non-reactive in the rapid immunochromatographic test.
Conclusion: Our study on three suspicious hunting dogs has suggested that Leishmania (Leishmaniainfantum may circulate both in endemic and non-endemic areas in Brazil. In addition, a high rate of hunting dog replacement due to death and trade may have led to less chance of infection and transmission between animals and between animals and humans, which would corroborate the outcomes reported here. Further studies should be conducted to fully establish whether hunting dogs and hunters may be used as sentinels in other areas endemic for Leishmania spp.

One Health approach on human seroprevalence of anti- Toxocara antibodiesToxocara spp. eggs in dogs and sand samples between seashore mainland and island areas of southern Brazil

  • Toxocariasis, caused by Toxocara spp. nematodes, is among the top 5 neglected parasitic diseases worldwide; however, no comprehensive study to date has serologically compared infections in people and their dogs and environmentally contaminated soil or sand of mainland and island locations. Accordingly, this study aimed to assess the seroprevalence of anti-Toxocara antibodies in traditional human seashore populations, the presence of eggs in dogs’ feces and hair, and the presence of eggs in environmental samples from islands compared to the adjacent mainland of southern Brazil. Overall, 212/328 (64.6%) people were positive for Toxocara spp. antibodies, including 125/190 (65.8%) island and 87/138 (63.0%) mainland residents.
  • For dog samples, 12/115 (10.43%) were positive for the presence of Toxocara spp. eggs, all from dogs living in islands, and 22/104 (21.15%) dog hair samples contained eggs of Toxocara spp. Environmental contamination with Toxocara spp. eggs was observed in 50/130 (38.46%) samples from all sampled sites. No significant association was found between risk factors (age, sex, educational level, monthly income, owning dogs or cats, ingestion of treated water, and consumption of raw or uncooked meat) and Toxocara spp. seropositivity.
  • The present study is the first concurrent report on people, their dogs, and environmental contamination of Toxocara spp. The high prevalence we observed in the seashore populations of both in island and mainland areas may be caused by exposure to contaminated sand and climatic factors favoring frequent exposure to Toxocara spp. In conclusion, seashore lifestyle and living conditions of both island and mainland areas may have predisposed higher contact with infected pets and contaminated soil, favoring the high prevalence of toxocariasis.

Motilin (Dog) antibody

Y120 Sceti 50 ul 427 EUR

Dog RBC antibody

20R-RR011 Fitzgerald 20 mg 327 EUR

Dog RBC antibody

20R-RR026 Fitzgerald 5 mg 554 EUR

DOG-1(DOG1.1) Antibody

BNC040725-100 Biotium 100uL 199 EUR

DOG-1(DOG1.1) Antibody

BNC040725-500 Biotium 500uL 544 EUR

DOG-1(DOG1.1) Antibody

BNC470725-100 Biotium 100uL 199 EUR

DOG-1(DOG1.1) Antibody

BNC470725-500 Biotium 500uL 544 EUR

DOG-1(DOG1.1) Antibody

BNCAP0725-100 Biotium 100uL 199 EUR

DOG-1(DOG1.1) Antibody

BNCAP0725-500 Biotium 500uL 544 EUR

DOG-1(DOG1.1) Antibody

BNCB0725-100 Biotium 100uL 199 EUR

DOG-1(DOG1.1) Antibody

BNCB0725-500 Biotium 500uL 544 EUR

DOG-1(DOG1.1) Antibody

BNC810725-100 Biotium 100uL 199 EUR

DOG-1(DOG1.1) Antibody

BNC810725-500 Biotium 500uL 544 EUR

Dog NT-proBNP Antibody

abx023577-1mg Abbexa 1 mg 1149 EUR

Dog NT-proBNP Antibody

abx023578-1mg Abbexa 1 mg 1261 EUR

DOG-1(DOG1.1) Antibody

BNC700725-100 Biotium 100uL 199 EUR

DOG-1(DOG1.1) Antibody

BNC700725-500 Biotium 500uL 544 EUR

DOG-1(DOG1.1) Antibody

BNC880725-100 Biotium 100uL 199 EUR

DOG-1(DOG1.1) Antibody

BNC880725-500 Biotium 500uL 544 EUR

DOG-1(DOG1.1) Antibody

BNC610725-100 Biotium 100uL 199 EUR

DOG-1(DOG1.1) Antibody

BNC610725-500 Biotium 500uL 544 EUR

DOG-1(DOG1.1) Antibody

BNC680725-100 Biotium 100uL 199 EUR

Anti-Brucella canis antibodies in dogs naturally infected with Leishmania infantum and associated histological alterations in the genital tract

In canine visceral leishmaniasis, coinfections can aggravate the disease. Our aim was to investigate Brucella canis in dogs infected with Leishmania infantum. One hundred and six L. infantum-seropositive dogs were submitted to serology for B. canis, PCR for B. canis and L. infantum, and histopathological analysis of the genital tract. Anti-B. canis antibodies were detected in seven dogs whose clinical signs, L. infantum load and histological alterations were similar to those of seronegative animals. The circulation of anti-B. canis antibodies was low but demonstrates the exposure of dogs to this bacterium in a visceral leishmaniasis-endemic area.

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